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KMID : 0364820180540020091
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Korean Journal of Microbiology
2018 Volume.54 No. 2 p.91 ~ p.97
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Effect of hnRNP-like protein THO4 on growth and mRNA export in fission yeast
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Park Jin-Hee
Lee So-Jeong
Yoon Jin-Ho
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Abstract
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The evolutionally conserved TREX complex member, Yra1/ALY,belongs to the REF (RNA and export factor binding proteins)family of hnRNP-like proteins, which has been implicated inmultiple processes including transcription, nuclear RNA stability,and mRNA export. Fission yeast, Schizosaccharomyces pombe,genome encodes two members of REF proteins. In addition toMlo3 known previously as an mRNA export factor, there isthe other REF protein, Tho4, which is predicted as a componentof THO complex. Here we showed that deletion of tho4(SPBC106.12c) gene does not inhibit both growth and nuclearmRNA export. However, overexpression of tho4 displays growthretardation and slight accumulation of poly(A)+ RNA in thenucleus. Neither Δtho4 Δmlo3 nor Δtho4 Δmex67 double mutantsexhibit additive growth defect. Moreover, yeast two-hybridand co-immunoprecipitation analysis did not show that theTho4 protein interacted with any members of TREX complexand mRNA export factor Rae1. Contrary to expectation, theseobservations support that the S. pombe Tho4 is not a component ofTREX complex, and not directly involved in bulk mRNAexport from the nucleus.
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KEYWORD
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Schizosaccharomyces pombe, mRNA export, Mlo3, Tho4, THO/TREX complex
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